Cholesterol (from the Ancient Greek chole- (bile) and stereos (solid), followed by the chemical suffix -ol for an alcohol) is an organic molecule. It is a sterol (or modified steroid), a type of lipid. Cholesterol is biosynthesized by all animal cells and is an essential structural component of animal cell membranes.
Cholesterol also serves as a precursor for the biosynthesis of steroid hormones, bile acid, and vitamin D. Cholesterol is the principal sterol synthesized by all animals. In vertebrates, hepatic cells typically produce the greatest amounts. It is absent among prokaryotes (bacteria and archaea), although there are some exceptions, such as Mycoplasma, which require cholesterol for growth. [Wikipedia]
The graph shows the SERS spectrum of 10-3 M aqueous solution of Cholesterol on S-Silver hydrophobic substrate.
Laser wavelength: 785 nm
Laser power: 20 mW
Integration time: 4 seconds
Raman Spectrometer: B&WTEK
Lens magnification: 50x
Sampling method: immersion
The substrate used: S-Silver hydrophobic (interchangeably, you can use one of our substrates)