Jan Paczesny, Łukasz Richter and Robert Hołyst
Bacteria will likely become our most significant enemies of the 21st century, as we are approaching a post-antibiotic era. Bacteriophages, viruses that infect bacteria, allow us to fight infections caused by drug-resistant bacteria and create specific, cheap, and stable sensors for bacteria detection. Here, we summarize the recent developments in the field of phage-based methods for bacteria detection. We focus on works published after mid-2017. We underline the need for further advancements, especially related to lowering the detection (below 1 CFU/mL; CFU stands for colony forming units) and shortening the time of analysis (below one hour). From the application point of view, portable, cheap, and fast devices are needed, even at the expense of sensitivity.
Keywords: bacteria detection; bacteriophages; phage-based sensors, blu-ray DVD, drop-casting, enhancement factor, rotavirus RNA, SERS, Paper-based SERS, Silver clusters, DFT, Experimental analysis, theoretical modelling, Raman, photoscience in biology, experimental techniques, Raman spectroscopy, theoretical approaches, single molecules, synthesis methods, ultrafast photochemistry, photoscience at nanoscale, infrared spectroscopy, surface enhanced raman spectroscopy, press, sciene, big scale research,