Sritam Biswas, Yengkhom Damayanti Devi, Dipjyoti Sarma, Nima D. Namsa, Pabitra Nath
Detection and estimation of various biomolecular samples are often required in research and clinical laboratory applications. Present work demonstrates the functioning of a surface-enhanced Raman scattering (SERS) substrate that has been obtained by drop-casting of citrate-reduced gold nanoparticles (AuNPs) of average dimension of 23 nm on a bare blu-ray digital versatile disc (BR-DVD) substrate. The performance of the proposed SERS substrate has been initially evaluated with standard Raman active samples, namely malachite green (MG) and 1,2-bis(4-pyridyl)ethylene (BPE). The designed SERS substrate yields an average enhancement factor of 3.2 106 while maintaining reproducibility characteristics as good as 94% over the sensing region of the substrate. The usability of the designed SERS substrate has been demonstrated through the detection and analysis of purified rotavirus double-stranded RNA (dsRNA) samples in the laboratory environment condition. Rotavirus RNA concentrations as low as 10 ng/μL could be detected with the proposed sensing scheme.
Keywords: blu-ray DVD, drop-casting, enhancement factor, rotavirus RNA, SERS, Paper-based SERS, Silver clusters, DFT, Experimental analysis, theoretical modelling, Raman, photoscience in biology, experimental techniques, Raman spectroscopy, theoretical approaches, single molecules, synthesis methods, ultrafast photochemistry, photoscience at nanoscale, infrared spectroscopy, surface enhanced raman spectroscopy, press, sciene, big scale research,